论文部分内容阅读
目的探讨柯萨奇 Ｂ组病毒抗体ＩｇＭ测定在心肌炎病原学诊断和临床诊断中的意义。方法１９９６～１９９８年儿科住院病人７０例，分为心肌炎组２０例，疑似心肌炎组２５例，对照组２５例。用间接酶联免疫吸附法（ＥＬＩＳＡ）测定柯萨奇Ｂ组病毒抗体ＩｇＭ和ＩｇＧ。结果柯萨奇Ｂ组病毒抗体ＩｇＭ阳性心肌炎组１１例（５５％），疑似心肌炎组１１例（４４％），对照组１１例（４４％），经统计学处理，其差别无显著性意义。结论用ＥＬＩＳＡ法测定柯萨奇Ｂ组病毒抗体ＩｇＭ，阳性提示有柯萨奇Ｂ组病毒感染，对心肌炎患儿可以作出病因和病原学诊断，但不能以此作为临床诊断心肌炎的依据。
Objective To investigate the significance of IgM determination of Coxsackie virus B in etiological diagnosis and clinical diagnosis of myocarditis. Methods From 1996 to 1998, 70 pediatric inpatients were divided into myocarditis group (n = 20), suspected myocarditis group (n = 25) and control group (n = 25). Coxsackie B virus IgM and IgG were determined by indirect enzyme-linked immunosorbent assay (ELISA). Results There were 11 cases (55%) of Coxsackie virus B (IgM) -positive myocarditis group, 11 cases (44%) of suspected myocarditis group and 11 cases (44%) of control group. The difference was not statistically significant. Conclusion ELISA method for the determination of Coxsackie B virus IgM, positive prompted Coxsackie B virus infection in children with myocarditis can make etiological and etiological diagnosis, but can not be used as a basis for the clinical diagnosis of myocarditis.