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目的:利用副结核杆菌接种地鼠,以图建立一个模拟克隆病的小动物模型。方法:将40只地鼠随机分为两组,实验组:20只腹腔接种副结核杆菌;对照组:20只分别接种生理盐水和死菌(各10只)。8个月后处死动物,作病理学观察,并对动物肠组织进行 Polymerase chain reaction(PCR)扩增副结核杆菌 DNA。结果:实验组 80% (16/ 20)的地鼠出现肉芽肿性肠炎。实验组所有 20只地鼠的肠组织均扩增出 400 bp的 DNA 片段。结论:建立了一个新的肉芽肿性肠炎的小动物模型。
Objective: Inoculation of M. gonorrhoeae with gonads in order to establish a model animal model of Crohn’s disease. Methods: Forty hamsters were randomly divided into two groups. In the experimental group, 20 M. tuberculosis strains were inoculated intraperitoneally. In the control group, 20 rabbits were inoculated with saline and dead bacteria (10 in each). Animals were sacrificed 8 months later for pathological observation, and animal intestinal tissue was subjected to Polymerase chain reaction (PCR) for amplifying M. tuberculosis DNA. Results: 80% (16/20) hamsters in the experimental group showed granulomatous enteritis. The intestinal tissues of all 20 hamsters in the experimental group amplified 400 bp DNA fragments. Conclusion: A new animal model of granulomatous enteritis was established.