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盆栽番茄Lycopersicon esculentum幼苗分别接种丛枝菌根(AM)真菌摩西球囊霉Glomus mosseae、地表球囊霉G.versiforme、根内球囊霉G.intraradices、幼套球囊霉G.etunicatum及珠状巨孢囊霉Gigaspora margarita 35d后,开始测定番茄植株内源信号物质水杨酸(SA)、茉莉酸(JA)、一氧化氮(NO)和过氧化氢(H2O2)含量变化,抗性相关酶活性,丙二醛(MDA)含量以及生长量等指标。结果表明,接种AM真菌增加了番茄植株鲜重、株高、地上部和地下部干重、叶片和根系NO、JA、H2O2含量和结合态SA含量,其中,以摩西球囊霉G.mosseae诱导作用最大,叶片和根系内NO、JA、H2O2和结合态SA含量分别比对照增加了3.3和1.9倍、6.8和8.0倍、0.9和1.2倍、1.9和2.6倍,而根系中游离态SA含量一直处于较低水平,只有摩西球囊霉G.mosseae处理在诱导高峰时根系游离态SA含量比对照略有增加。接种AM真菌处理的番茄叶片和根系超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)活性显著增加,其中以摩西球囊霉G.mosseae的诱导效应最大,与未接种对照相比分别增加了0.6和0.3倍、7.9和3.1倍、0.4和1.2倍、2.3和1.9倍;幼套球囊霉G.etunicatum的诱导效应最小:与未接种对照相比分别增加了0.26和0.14倍、2.3和1.0倍、0.1和0.28倍、0.55和0.31倍;而MDA含量下降,分别降低了66%和68%、34%和41%、51%和50%、12%和26%、18%和29%。表明AM真菌能诱导植物同时产生多种信号物质,而且这些信号参与了AM真菌-番茄共生体系统抗性的表达。
Potted tomato Lycopersicon esculentum seedlings were inoculated with arbuscular mycorrhizal (AM) fungi Glomus mosseae, G. terrestris, G. intraradices, G.etunicatum and Beads After 35 days of Gigaspora margarita, the contents of SA, JA, NO and H2O2, endogenous signal components of tomato plants, Activity, malondialdehyde (MDA) content and growth and other indicators. The results showed that the inoculation of AM fungi increased the fresh weight, plant height, dry weight of shoots and roots, NO, JA, H2O2 content and bound SA content in leaves and roots of AM plants. Among them, G. mosseae The contents of NO, JA, H2O2 and bound SA in leaves and roots increased by 3.3 and 1.9 times, 6.8 and 8.0 times, 0.9 and 1.2 times, 1.9 and 2.6 times respectively, while the content of free SAs in roots was always at At a lower level, only S. grisea G.mosseae had slightly higher levels of free radical SA at root induction than the control. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and phenylalanine ammonia lyase (PAL) in tomato leaves and roots treated with AM fungi were significantly increased The induction effect of G. mosseae was the largest with an increase of 0.6 and 0.3 fold, 7.9 and 3.1 fold, 0.4 and 1.2 fold, 2.3 and 1.9 fold respectively compared with the unvaccinated control; Induction effects were minimal: 0.26 and 0.14 fold, 2.3 and 1.0 fold, 0.1 and 0.28 fold, 0.55 and 0.31 fold respectively compared with unvaccinated controls; whereas MDA levels decreased by 66% and 68% and 34% and 41%, 51% and 50%, 12% and 26%, 18% and 29%. This indicated that AM fungi could induce plants to produce many kinds of signal substances at the same time, and these signals involved in the AM fungal-tomato symbiont system expression.