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近年来,国外采用液态氮超低温法保藏某些冷冻真空干燥法不易保藏成活的菌种而获得了满意的结果。其具体做法是:将已封口的菌种安瓿管置入慢速冻结器内,在控制速度为每分钟下降1℃的条件下,使样品冻结到-35℃,然后立即放入液氮容器中保藏。我处应用液态氮保藏草菇菌种时,由于缺少慢速冻结器,而采用一步速投法同样获得较理想的结果,克服了草菇常温保藏多次转管的缺点。
In recent years, foreign countries using liquid nitrogen cryogenic preservation of some frozen vacuum drying method is not easy to preserve the survival of strains obtained satisfactory results. The specific practice is: the sealed ampoule ampoule placed in a slow-freezing device at a controlled rate of decline of 1 ℃ per minute, the sample was frozen to -35 ℃, and then immediately placed in a liquid nitrogen container preservation. My application of liquid nitrogen preservation mushroom strains, due to the lack of slow-freezing, and the use of one-step vote cast method also get better results, to overcome the shortcomings of mushroom preservation at room temperature several times.