目的建立神经胶质瘤双向电泳技术。方法利用不同体积的裂解液提取大鼠脑组织中的蛋白质,并通过不同的蛋白质上样量(1、2、3mg),进行双向电泳,考马斯亮蓝染色,图谱分析。结果在等电点3～10,相对分子质量6.5～200KDa范围内分离得到蛋白质斑点为,1mg蛋白质上样量为574个蛋白质斑点,2mg为798个,3mg为803个斑点。2mg蛋白质上样量的双向电泳图谱更清晰,分离更好。结论成功建立了神经胶质瘤蛋白质的双向电泳技术。 Objective To establish a two-dimensional gel electrophoresis technique for glioma. Methods Different concentrations of lysate were used to extract protein from rat brain. Two different electrophoresis samples (1, 2, 3 mg) were used for two-dimensional electrophoresis, Coomassie brilliant blue staining and pattern analysis. Results The protein spots were separated in the range of 3 ~ 10 isoelectric point and 6.5 ~ 200KDa molecular weight, 1mg protein sample was 574 protein spots, 2mg 798, 3mg 803 spots. Two-dimensional electrophoresis pattern of 2mg protein sample is clearer and the separation is better. Conclusion Two-dimensional gel electrophoresis of glioma proteins has been successfully established.