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目的：从鸡蛋中提取特异性抗体，鉴定抗体性质并分析其蛋白质含量，以探索一种制备被动免疫防龋抗体的制备方法。方法：变形链球菌 GTF高表达株S.mutans B29-33 及茸毛链球菌S.sobrinus 6715免疫母鸡，采用硫酸铵沉淀法提取鸡蛋黄抗体，SDS-PAGE凝胶扫描鉴定抗体性质，分析其纯度并用分光光度法测蛋白质含量。结果：用硫酸铵沉淀法可提取出鸡蛋黄抗体，其性质为IgG，蛋白质含量约为7.00mg/ml。结论：鸡蛋黄抗体含量高，作为被动免疫抗体的载体有较好的应用前景。“,”Objective：The specific antibody was isolated from egg yolk in order to find a new method for passive immunization against dental caries. Methods：50 hens were divided into two groups and were injected with S.mutans B29-33 and S.sobrinus 6715 whole cells respectively. Egg yolk antibody IgY were isolated by sodium sulfate precipitating method and identified by SDS-PAGE. After electrophoresis the gel was scanned by chromatographic scanner and the protein content was evaluated by absorption spectrometry. Results： SDS-PAGE and gel scanner revealed the substance isolated from egg yolk was IgG. The protein content was 7.00mg/ml. Conclusion：Egg yolk antibody was isolated conveniently and the protein content was high. So it can be use as a suitable source of antibody for passive immunization.