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小麦赤霉菌 Gibberella zeae(Schw.)Petch 在麦粒培养基上进行固体产毒培养,从中提取粗毒素。用粗毒素溶液处理小麦抗感赤霉病不同品种萌动—致的种子以及—叶期幼根和叶鞘。用 DDS-11A 型电导仪测定细胞渗透性变化。结果表明,在粗毒素作用下,小麦根、芽生长受抑制。但抗病品种受抑轻,感病品种受抑重。抗感品种幼根和叶鞘细胞渗透性的变化差异明显。用赤霉菌粗毒素测定细胞渗透性的变化,有可能作为鉴定小麦品种抗赤霉病的一个生理指标。
Gibberella zeae (Schw.) Petch was subjected to solid toxigenic culture on wheat kernel medium from which crude toxins were extracted. Treatment of germinated seeds of different varieties of wheat with resistance to Fusarium headstone with crude toxin solution and shoots and leaf sheaths at leaf stage. Cell permeability changes were measured using a DDS-11A conductivity meter. The results showed that under the action of crude toxin, the growth of wheat roots and buds was inhibited. However, the disease-resistant varieties were depressed, susceptible varieties were suppressed. There were significant differences in the permeability of root and sheath among resistant varieties. Determining the change of cell permeability with the crude toxin of scab could be used as a physiological index to identify the scab resistance of wheat cultivars.