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目的 探索日本血吸虫 (中国大陆株 ) 2 2 .6ku抗原 ( Sj2 2 .6)编码基因用作核酸疫苗的可行性。方法 用新设计的引物以 PCR法对 Sj2 2 .6编码基因进行改造 ,然后亚克隆入真核表达载体 p CMV -β并转化入大肠杆菌 JM 10 9。结果 提取重组质粒经酶切分析 ,筛选出了含有正向插入片段的阳性重组质粒。结论 此阳性重组质粒可用于核酸疫苗等进一步研究
Objective To explore the feasibility of using 22 ku antigen (Sj2 2 .6) gene of Schistosoma japonicum (Chinese mainland strain) as nucleic acid vaccine. Methods The Sj2 2. 6 gene was modified by PCR with the newly designed primers and then subcloned into the eukaryotic expression vector pCMV-β and transformed into E. coli JM109. Results Recombinant plasmids were extracted and digested with restriction endonucleases. The positive recombinant plasmids containing the positive insert were screened out. Conclusion This recombinant plasmid can be used for further study of nucleic acid vaccine