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研究了抗癌导向药物IgY-Ricin A杀伤癌细胞的作用机理。人胃低分化粘液腺癌MGC-803细胞经IgY-Ricin A处理,细胞的增殖明显受到抑制,而同样处理的人胚正常肺细胞2BS,其生长不受这种药物的影响。FCM实验结果表明,IgY-Ricin A处理的MGC-803细胞,在8h开始出现细胞程序化死亡峰Apo,同样处理的2BS细胞则无Apo峰出现。经lgY-Ricin A处理的MGC-803细胞核由均一状态变为浓缩凝集状,经激光共聚焦显微镜进行光切片和三维重组,发现MGC-803细胞核由原来的球形变成高度浓缩凝集的点状结构。DNA凝胶电泳分析显示,IgY-Ricin A处理的MGC-803细胞DNA被降解,呈现梯状电泳条带这一典型的细胞程序化死亡指标。研究结果表明,IgY-Ricin A通过诱导细胞程序化死亡来控制MGC-803细胞的增殖,最终杀死癌细胞。
The mechanism of anti-cancer drug IgY-Ricin A killing cancer cells was studied. When human gastric poorly differentiated mucinous adenocarcinoma MGC-803 cells were treated with IgY-Ricin A, the proliferation of the cells was significantly inhibited. However, the same treatment of human embryonic normal lung cells 2BS was not affected by this drug. The results of FCM experiments showed that the programmed cell death peak Apo began to appear in IgY-Ricin A-treated MGC-803 cells at 8 h, and no Apo peak appeared in the same 2BS cells. The nucleus of MGC-803 treated with lgY-Ricin A changed from homogenous state to concentrated agglutinated state, and the sections of MGC-803 cell nucleus changed from original sphere to highly condensed agglutinated dot structure by laser confocal microscopy. . DNA gel electrophoresis analysis showed that DNA of IgY-Ricin A-treated MGC-803 cells was degraded, showing a ladder-like electrophoretic band, a typical programmed cell death index. The results showed that IgY-Ricin A controls the proliferation of MGC-803 cells by inducing programmed cell death and ultimately kills cancer cells.