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在甲酸(α相)和氢氧化钠(γ相)缓冲液形成的移动反应界面的基础上,提出了一种衍生移动反应界面模型。模型表明在α相和γ相之间会形成一个新的β相,β相和α相形成衍生移动反应界面,β相和γ相形成移动界面。为了验证该模型的有效性,该文给出了相关理论及数值推导过程。此外,基于毛细管电泳和自制装置进行了相关实验。结果表明,若使用以前的移动反应界面,实验结果与理论计算存在较大误差,而采用该文提出的衍生移动反应界面,实验数据与理论计算结果高度一致。该文提出的衍生移动反应界面理论及模型对于电泳,特别是毛细管电泳中样品的分离与富集具有重要的意义。
Based on the mobile reaction interface formed by formic acid (α phase) and sodium hydroxide (γ phase) buffer, a derivative mobile reaction interface model was proposed. The model shows that a new β phase is formed between the α phase and the γ phase. The β phase and the α phase form a derivative mobile reaction interface, and the β phase and the γ phase form a moving interface. In order to verify the validity of the model, this paper gives the relevant theory and numerical derivation process. In addition, experiments based on capillary electrophoresis and home-made devices were performed. The results show that if the previous mobile reaction interface is used, there is a big error between experimental results and theoretical calculation, and the derived mobile reaction interface is adopted. The experimental data are in good agreement with the theoretical calculation results. The derived mobile reaction interface theory and model are of great significance to the separation and enrichment of samples in electrophoresis, especially in capillary electrophoresis.