目的:通过代谢组学的方法寻找系统性红斑狼疮(SLE)小鼠血清中的差异代谢物,以研究SLE所引起的机体代谢通路变化,探讨SLE的发病机制。方法:分别应用高效液相色谱-四级杆-飞行时间质谱联用系统的正离子、负离子模式分析小鼠的血清样本,使用有监督的偏最小二乘-判别分析研究对照组和SLE模型组之间的代谢组学差异,寻找差异代谢物,并使用精确质量数结合二级质谱鉴定差异代谢物。结果:寻找并鉴定了12(S)-HETE,EPA,Hepoxilin B3等12个差异代谢物。这些差异代谢物大多与不饱和脂肪酸和氨基酸的代谢有关。结论:SLE发病对体内不饱和脂肪酸和氨基酸代谢通路有显著的影响。部分差异代谢物有调节免疫的作用,体内不饱和脂肪酸和氨基酸的代谢紊乱,可能会加重SLE发病时全身性的炎症反应。 OBJECTIVE: To find the differential metabolites in the serum of systemic lupus erythematosus (SLE) mice by means of metabonomics to study the changes of metabolic pathways in the body caused by SLE and to explore the pathogenesis of SLE. Methods: Serum samples of mice were analyzed by positive ion and negative ion mode respectively using high performance liquid chromatography - quadrupole - time of flight mass spectrometry. Supervised least square - discriminant analysis was used to study the control group and SLE model group Metabonomics differences between the search for differential metabolites, and the use of accurate mass combined with secondary mass spectrometry identification of differential metabolites. Results: 12 differential metabolites such as 12 (S) -HETE, EPA and Hepoxilin B3 were identified and identified. Most of these differential metabolites are related to the metabolism of unsaturated fatty acids and amino acids. Conclusion: The pathogenesis of SLE has a significant effect on the unsaturated fatty acid and amino acid metabolic pathways in vivo. Part of the differential metabolites have the role of immune regulation, metabolic disorders of unsaturated fatty acids and amino acids in vivo may aggravate the systemic inflammatory response in the pathogenesis of SLE.