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本研究旨在检测肿瘤抑制基因PTEN (phosphatase and tensin homolog deleted on chromosome ten)在早孕小鼠子宫内膜中的表达规律,探讨PTEN 在小鼠胚胎着床过程中的作用。采用实时荧光定量聚合酶联反应(real-time fluorescent quantitative PCR,FQ-PCR)和免疫组织化学方法分别检测未孕及孕 1、3、4、5、7 d 小鼠子宫内膜 PTEN mRNA 和蛋白的表达;子宫角注射PTEN反义寡核苷酸观察胚泡着床数。FQ-PCR结果显示,妊娠小鼠子宫内膜组织PTEN mRNA的表达高于未妊娠小鼠,且随着妊娠天数的增加表达逐渐增强,到妊娠第 5 天达最高。免疫组织化学分析显示,PTEN 蛋白在子宫内膜的表达规律与mRNA 结果一致。子宫角注射 PTEN 反义寡核苷酸后胚泡着床数明显减少。结果提示,PTEN 在妊娠早期子宫内膜持续表达,可能参与了胚泡着床。
This study aimed to investigate the expression of PTEN (phosphatase and tensin homolog deleted on chromosome ten) in the endometrium of early pregnancy mice and to explore the role of PTEN in mouse embryo implantation. The endometrial PTEN mRNA and protein in non-pregnant and gestational 1, 3, 4, 5 and 7 days mice were detected by real-time fluorescent quantitative PCR (FQ-PCR) and immunohistochemistry The expression of blastocyst was observed by injecting PTEN antisense oligonucleotide into the uterus. The results of FQ-PCR showed that the expression of PTEN mRNA in endometrium of pregnant mice was higher than that of non-pregnant mice, and gradually increased with the increase of gestational days, reaching the highest on the 5th day of pregnancy. Immunohistochemical analysis showed that PTEN protein expression in the endometrium consistent with the mRNA results. After injection of PTEN antisense oligonucleotide into uterine horn, the number of blastocyst implantation significantly decreased. The results suggest that, PTEN in early pregnancy endometrial continuous expression may be involved in blastocyst implantation.