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目的观察健脾化湿法对特应性皮炎(AD)小鼠水通道蛋白3(AQP 3)表达的影响,探讨健脾化湿法治疗AD的作用机制与途径,为进一步阐明健脾化湿法治疗AD的分子生物学提供依据。方法将30只7周龄昆明小鼠随机分为中药模型组、空白模型组、空白对照组,每组各10只。中药模型组和空白模型组建立皮炎模型,建模后中药模型组采用小儿健脾化湿水煎液灌胃,剂量为10 m L/kg,2次/d,空白模型组以等量生理盐水灌胃;空白对照组不建立皮炎模型,以等量生理盐水灌胃。小鼠鼠龄达11周龄同时取其背部皮肤组织采用免疫组织化学法(PV法)检测AD小鼠皮肤AQP 3的表达及分布。结果中药模型组、空白模型组、空白对照组小鼠皮肤组织中AQP 3吸光度值分别为0.450±0.061、0.611±0.063、0.261±0.040,空白模型组高于空白对照组,差异有统计学意义(P<0.01);中药模型组低于空白模型组,差异有统计学意义(P<0.01)。结论健脾化湿法可以明显改善AD小鼠皮肤组织中AQP 3的表达与分布,促进皮肤组织的修复。
Objective To observe the effect of invigorating the spleen and dampness method on the expression of aquaporin 3 (AQP 3) in mice with atopic dermatitis (AD) and to explore the mechanism and ways of treating the AD with the method of invigorating the spleen and removing dampness. In order to further elucidate the mechanism of spleen- Law to provide the basis for the molecular biology of AD. Methods Thirty Kunming mice of 7 weeks old were randomly divided into TCM model group, blank model group and blank control group, with 10 mice in each group. The Chinese medicine model group and the blank model group were established dermatitis model. After the model was established, the Chinese medicine model group was treated by infantile invigorating spleen and dampness decoction orally at a dose of 10 m L / kg twice a day. The blank model group was treated with an equal volume of normal saline Gavage; blank control group does not establish dermatitis model, with the same amount of normal saline gavage. At the age of 11 weeks, mice were sacrificed and the skin of the back was removed. The expression and distribution of AQP 3 in the skin of AD mice were detected by immunohistochemistry (PV method). Results The AQP 3 absorbance values of the skin tissue in the model group, the blank model group and the blank control group were 0.450 ± 0.061, 0.611 ± 0.063 and 0.261 ± 0.040, respectively, which were significantly higher in the blank model group than in the blank control group (the difference was statistically significant ( P <0.01). The Chinese medicine model group was lower than the blank model group, the difference was statistically significant (P <0.01). Conclusion The method of invigorating the spleen and dampness can obviously improve the expression and distribution of AQP 3 in the skin of AD mice and promote the repair of skin tissue.