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目的:观察结肠粘膜固有层淋巴细胞(LPL)凋亡与溃疡性结肠炎的关系,并进一步探讨清肠栓对溃疡性结肠炎结肠粘膜LPL的影响。方法:SD大鼠随机分为正常组、模型组、西药组、中药组,用5%2,4,6三硝基苯璜酸100mg.kg-1灌肠建模,3天后开始,除正常组外,各组分别给予生理盐水、柳氮磺胺吡啶、清肠栓处理,至7天后处死动物。分别运用电镜、流式细胞仪碘化丙啶法(PI染色法)、原位细胞凋亡检测法,来检测溃疡性结肠炎大鼠结肠粘膜LPL凋亡。结果:模型组结肠粘膜LPL凋亡减慢,中、西药组均可诱导结肠粘膜LPL凋亡,中药组与模型组相比,P<0.05。结论:结肠粘膜LPL的减慢和溃疡性结肠炎的发生存在一定关系,清肠栓可能通过诱导结肠粘膜LPL凋亡达到缓解溃疡性结肠炎的目的。
OBJECTIVE: To observe the relationship between colonic mucosal lamina propria lymphocyte (LPL) apoptosis and ulcerative colitis, and further investigate the effect of Qingchang suppository on LPL in colonic mucosa of ulcerative colitis. METHODS: Sprague-Dawley rats were randomly divided into normal group, model group, western medicine group, and traditional Chinese medicine group. They were modeled with 5% 2,4,6-trinitrophenylhydrazone 100 mg.kg-1 enema and started 3 days later, except for normal group. In addition, each group was given physiological saline, sulfasalazine, and clear bowel suppositories, and animals were sacrificed 7 days later. Electron microscopy, flow cytometry propidium iodide staining (PI staining), and in situ cell apoptosis assay were used to detect the apoptosis of colonic mucosal LPL in rats with ulcerative colitis. Results: The apoptosis of LPL in the colonic mucosa of the model group was slowed down, and the apoptosis of LPL in the colonic mucosa was induced in the Chinese and western medicine groups. Compared with the model group, the Chinese medicine group had P<0.05. Conclusion: There is a certain relationship between the decrease of LPL in colon mucosa and the occurrence of ulcerative colitis. Qingchang suppository may relieve ulcerative colitis by inducing apoptosis of colonic mucosal LPL.