神经生长因子和增殖细胞核抗原在人脑胶质瘤中的表达及意义

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目的:研究人脑胶质瘤中神经生长因子(NGF)和增殖细胞核抗原(PCNA)的表达,探讨它们与胶质瘤病理分级的关系及其在评估胶质瘤患者预后中的作用。方法:采用SP免疫组织化学法检测10例正常脑组织和64例胶质瘤中NGF和PCNA的表达,并分析其在正常脑组织及不同级别胶质瘤间的差异。结果:在正常脑组织与胶质瘤之间,以及高级别胶质瘤组与低级别胶质瘤组之间,NGF和PCNA的表达均存在显著差异(P<0.05),且随肿瘤病理级别的升高而增高;两者在生存时间>2年组与生存时间≤2年组之间的差别显著(P<0.05);相关性分析显示二者的表达存在正相关。结论:NGF和PCNA在胶质瘤的发生、发展过程中可能起重要的作用,其表达水平高低与胶质瘤的恶性程度及患者预后密切相关。 OBJECTIVE: To study the expression of nerve growth factor (NGF) and proliferating cell nuclear antigen (PCNA) in human glioma and to explore their relationship with the pathological grade of glioma and its role in evaluating the prognosis of patients with glioma. Methods: The expression of NGF and PCNA in 10 cases of normal brain tissue and 64 cases of gliomas were detected by SP immunohistochemistry. The difference between normal brain tissue and different grades of gliomas was analyzed. Results: The expression of NGF and PCNA were significantly different between normal brain tissue and glioma, as well as between high-grade glioma group and low-grade glioma group (P <0.05), and with the tumor pathological grade (P <0.05). The correlation analysis showed that there was a positive correlation between the two expression levels. Conclusion: NGF and PCNA may play an important role in the development and progression of glioma. The expression level of NGF and PCNA is closely related to the malignant degree and prognosis of gliomas.
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