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本文应用Howell银胶染色法对39例病检资料(其中BCA 14例、ACC22例、正常涎腺3例)进行AgNORs定量分析。实验结果表明:ACC组细胞内AgNORs较多,平均每个细胞为2.68±0.7,个别可多达6～8;BCA组平均每个细胞为1.34±0.098,个别3～4;正常涎腺导管和腺泡细胞平均为1.23±0.04,个别亦为3～4。后两者与前者间的差异在统计学上有显著性意义(P<0.01)。因此,我们认为应用银胶染色法进行AgNORs定量分析有助于区分BCA和ACC,有一定的实用意义。另对本组22例ACC按其组织学类型进行AgNORs定量分析,发现14例分化较好的筛状型和管状型ACC的AgNORs数明显低于8例分化较差的实体型和混合型ACC,两者在统计学上有显著性差异(P<0.01)。
In this paper, Howell colloidal silver staining method for 39 cases of pathological data (including BCA in 14 cases, ACC22 cases, normal salivary glands in 3 cases) AgNORs quantitative analysis. The results showed that there were many AgNORs in ACC group, with an average of 2.68 ± 0.7 per cell and up to 6 ~ 8 in each cell group. The average number of cells per cell in BCA group was 1.34 ± 0.098, with 3 ~ 4 in each group. The average number of acinar cells was 1.23 ± 0.04, and the individual was also 3-4. The difference between the latter two and the former was statistically significant (P <0.01). Therefore, we think that AgNORs quantitative analysis with silver colloid staining method can help distinguish between BCA and ACC, which is of practical significance. Another 22 cases of this group of ACC according to their histological type of AgNORs quantitative analysis found that 14 well-differentiated mesh-like and tubular ACC AgNORs number was significantly lower than 8 poorly differentiated solid and mixed ACC, two There was a statistically significant difference (P <0.01).