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目的:分析子宫内膜异位症(内异症)患者血清中抗子宫内膜抗体(EMAb)的特性并探讨其对内异症的诊断价值。方法:应用免疫印迹法(western blot)对内异症患者在位及异位内膜抗原蛋白进行分析,并检测内异症患者血清中针对这些抗原的EMAb。结果:在位子宫内膜与异位子宫内膜抗原识别条带有差异。与内异症患者血清发生反应的异位抗原条带主要为36kD,而在位抗原条带主要为60kD。免疫印迹法检测EMAb的敏感度为71.4%,特异性为84.3%。结论:免疫印迹法检测内异症患者血清中的EMAb具有较好的敏感度和特异性。与EMAb产生有关的异位内膜抗原的提纯和克隆将为内异症的临床诊断提供更为有效的方法。
Objective: To analyze the characteristics of anti-endometrial antibody (EMAb) in serum of patients with endometriosis (endometriosis) and to explore its diagnostic value of endometriosis. Methods: Western blotting was used to analyze eutopic and ectopic endometrial protein in patients with endometriosis and to detect EMAb against these antigens in sera of patients with endometriosis. Results: There were differences between eutopic endometrium and ectopic endometrial antigens recognition bands. The ectopic antigen bands that reacted with the serum of endometriosis patients were predominantly 36 kD, while the predominant antigen bands were predominantly 60 kD. The sensitivity and specificity of EMAb by immunoblotting were 71.4% and 84.3% respectively. CONCLUSIONS: Western blotting for the detection of EMAb in sera of patients with endometriosis has good sensitivity and specificity. Purification and cloning of ectopic endometrial antigens associated with EMAb production will provide a more effective method for the clinical diagnosis of endometriosis.