论文部分内容阅读
观察编码鼠IL - 12的真核表达载体对HBVDNA疫苗诱导Balb/c小鼠免疫应答的影响。方法 :肌肉注射DNA疫苗 ,ELISA法检测小鼠血清抗HBs,4h51Cr释放法检测小鼠脾细胞CTL杀伤活性。结果 :免疫8周后 ,注射 pCR3.1组、注射 pCR3.1-S组及共注射IL - 12真核表达载体的血清OD值分别为 0 .0 4± 0 .0 1、0 .87± 0 .1及 1.6 7± 0 .15。CTL细胞杀伤活性分别为 (10 .1± 6 .4) %、(5 0 .5± 6 .4) %及 (73.3± 8.8) % ,后两组与pCR3.1组比较均有显著差异 (P <0 .0 0 1) ,后两组比较亦有显著差异 (P <0 .0 5 )。脾细胞悬液经抗CD4+ 单克隆抗体处理后分别为 (48.3± 5 .9) %、(75 .6± 9.1) % ,抗CD8+ 单克隆抗体处理后分别为 (10 .6±1.4) %、(16 .9± 2 .3) %。结论 :IL - 12的真核表达载体能够提高小鼠对DNA疫苗的免疫应答 ,CTL细胞杀伤活性主要由CD8+ 执行。
To observe the effect of eukaryotic expression vector encoding murine IL - 12 on immune response induced by HBVDNA vaccine in Balb / c mice. Methods: DNA vaccine was intramuscularly injected into mice. Serum anti-HBs were detected by ELISA and cytotoxicity against CTL was detected by 4h51Cr release assay. Results: After 8 weeks of immunization, the serum OD value of pCR3.1 injection group, pCR3.1-S injection group and co-injection of IL-12 eukaryotic expression vector were respectively 0.040 ± 0.010 0 .1 and 1.6 7 ± 0 .15. The cytotoxicity of CTL was (10.1 ± 6.4)%, (50.5 ± 6.4)% and (73.3 ± 8.8)%, respectively. There were significant differences between the latter two groups and the pCR3.1 group P <0.01), there was also a significant difference between the two groups (P <0.05). The splenocytes suspension were (48.3 ± 5.9)% and (75.6 ± 9.1)% when treated with anti-CD4 + monoclonal antibody and (10.6 ± 1.4)% and (16.9 ± 2.3%). Conclusion: The eukaryotic expression vector of IL - 12 can enhance the immune response to DNA vaccine in mice. The cytotoxic activity of CTL is mainly mediated by CD8 +.