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目的:观察磨盘草石油醚部位对异丙肾上腺素(ISO)致大鼠心力衰竭的改善并分析可能机制。方法:采用腹腔注射ISO(2mg/kg)4周的方法建立大鼠心衰模型,后分组进行灌胃给药治疗,连续4周。采用放射性免疫法测定大鼠血清中心钠肽(ANP)、血管紧张素Ⅱ(AngⅡ)、醛固酮(Ald)水平,并取心脏组织称重记录,计算心脏指数,观察各组大鼠的心肌病理形态学特征。结果:与正常对照组比较,模型对照组心脏指数明显增加(P<0.01),及血清中ANP、AngⅡ、ALD均显著升高(P<0.05,P<0.01),心肌纤维排列紊乱,血管扩张,可见小灶性纤维瘢痕组织;与模型对照组比较,磨盘草石油醚部位各剂量组血清中指标均出现显著降低(P<0.05,P<0.01)。心肌病理形态学特征方面,低、中剂量组相比不明显,高剂量组有所改善。结论:磨盘草石油醚部位可改善ISO所致的大鼠心衰,其机制可能与降低血清中的ANP、AngⅡ、Ald的水平相关。
OBJECTIVE: To observe the effects of petroleum ether fraction on the isoproterenol (ISO) -induced heart failure in rats and its possible mechanism. Methods: The rat model of heart failure was established by intraperitoneal injection of ISO (2mg / kg) for 4 weeks. The rats in each group were given gavage for 4 weeks. Radioimmunoassay was used to determine the levels of serum atrial natriuretic peptide (ANP), angiotensin Ⅱ (AngⅡ) and aldosterone (Ald) in rats. The cardiac tissue was weighed, and the cardiac index was calculated. Learn characteristics. Results: Compared with the normal control group, the cardiac index of the model control group increased significantly (P <0.01) and the levels of ANP, AngⅡ and ALD in the model group increased significantly (P <0.05, P <0.01) (P <0.05, P <0.01). Compared with the model control group, the indexes of serum in the different doses of petroleum ether fraction in the disc were significantly decreased (P <0.05, P <0.01). Myocardial pathological features, low, medium dose group was not obvious, high-dose group improved. Conclusion: The petroleum ether fraction of Coprinus comatus can improve ISO-induced heart failure in rats. The mechanism may be related to the decrease of serum ANP, AngⅡ and Ald levels.