本文对银杏子叶愈伤组织进行了超低温保存研究。结果表明 ,冰冻保护剂、冷冻速度对解冻后材料相对存活率影响较大 ,冻前预培养和预冻至某一温度停留一段时间也较为重要。在含高浓度山梨醇或甘露醇的培养基上预培养 2d的愈伤组织 ,用 10 %二甲基亚砜 +0 .5mol·L- 1 山梨醇作为冰冻保护剂 ,以 1℃·min- 1 速度降温 ,在 - 15℃、- 35℃分别停留 10min和 30min后 ,投入液氮中保存 1d ,细胞相对存活率可达 6 0 %以上。再培养时 ,细胞能恢复生长。 In this paper, ginkgo cotyledons were cryopreserved. The results show that the freezing protective agent and freezing speed have a great influence on the relative survival rate of thawed materials. It is also important to pre-freeze and pre-freeze to a certain temperature for a period of time before freezing. The callus pre-cultured for 2 days on medium containing high concentration of sorbitol or mannitol was treated with 10% dimethyl sulfoxide +0.5 mol·L-1 sorbitol as cryoprotectant at 1 ℃ · min- 1 speed cooling, at -15 ℃, - 35 ℃ respectively stay 10min and 30min, into liquid nitrogen storage 1d, the relative survival rate of cells up to 60%. When cultured, the cells regrow.