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目的:探讨抗神经生长因子抗体(anti-NGF)对改善子宫腺肌病(AM)小鼠宫腔内环境的作用。方法:将15只雌性ICR小鼠随机分为AM治疗组(A组)、AM溶剂对照组(B组)及正常对照组(C组),A、B组使用口服他莫昔芬法建立AM小鼠模型。A组小鼠腹腔注射anti-NGF,B组以等体积的生理盐水替代,C组不作任何处理。3组小鼠于动情前期处死取双子宫称量后检测缓激肽受体1(BKR-1)、神经激肽1受体(NK-1R)、同源框基因转录因子A10(HOXA10)及芳香化酶(aromatase)基因与蛋白的表达。结果:A组子宫相对重量同B组,且均明显高于C组(P<0.05);A组BKR-1基因及蛋白和NK-1R基因水平较B组明显降低(P<0.05),与C组比较无统计学差异(P>0.05);A组HOXA10基因及蛋白水平较B组明显增高(P<0.05),与C组比较无统计学差异(P>0.05);A组芳香化酶基因及蛋白水平较B组减低(P<0.05),但仍高于C组(P<0.05)。结论:anti-NGF可显著缓解AM模型小鼠子宫炎症,改善内膜容受性、抑制芳香化酶表达,但短期使用不能抑制病灶增殖。
Objective: To investigate the effect of anti-NGF on improving uterine environment of adenomyosis (AM) mice. Methods: Fifteen female ICR mice were randomly divided into AM group (AM group), AM solvent control group (B group) and normal control group (C group). Groups A and B received oral tamoxifen to establish AM Mouse model. Group A mice were injected intraperitoneally with anti-NGF, group B was replaced by an equal volume of saline, and group C was given no treatment. Three groups of mice were sacrificed at the early estrus stage to measure the expression of bradykinin receptor 1 (BKR-1), neurokinin 1 receptor (NK-1R), and homeobox gene transcription factor A10 (HOXA10) Aromatase gene and protein expression. Results: The relative weight of the uterus in group A was significantly higher than that in group B (all P <0.05), and the levels of BKR-1 gene and protein and NK-1R in group A were significantly lower than those in group B (P <0.05) There was no significant difference between group C and group C (P> 0.05); The HOXA10 gene and protein level in group A was significantly higher than that in group B (P <0.05) The level of gene and protein was lower than that of group B (P <0.05), but still higher than that of group C (P <0.05). Conclusion: Anti-NGF can significantly alleviate uterine inflammation, improve endometrial receptivity and inhibit aromatase expression in AM model mice, but short-term use can not inhibit the proliferation of mice.