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Aim:To investigate whether IL-12p40 plays a crucial role in regulating islet al-lograft rejection in a streptozotocin(STZ)-induced diabetes mouse model.Methods:C57BL/6 and IL-12p40 gene knockout mice were selected as recipient mice,towhich the diabetes was induced with a treatment of STZ(150-200 mg/kg)by asingle ip injection.BALB/c mice were selected as donor mice and islet cells wereisolated from the mice.The 500 islets were transplanted into recipient mice be-neath the capsule of the left kidney.Following the islet transplantation the glu-cose from the mice sera was monitored and the rejection rate of islets was analyzed.Results:STZ could induce diabetes in the recipient mice within 1 week.Aftertransplantation of allograft islets,the increased glucose in wild-type(WT)micereturned to normal level and was maintained for 10 d.Unexpectedly,the rejectionrate of islet allograft between IL-12p40-deficient mice and WT mice was similar.Conclusion:The results suggested that,although islet allograft rejection is be-lieved to be Th1-cell predominant,the Th1 response inducer,IL-12 and IL-23 arenot essential to induce islet allograft rejection.
Aim: To investigate whether IL-12p40 plays a crucial role in regulating islet al-lograft rejection in a streptozotocin (STZ) -induced diabetes mouse model. Methods: C57BL / 6 and IL-12p40 gene knockout mice were selected as recipient mice, towhich the diabetes was induced with a treatment of STZ (150-200 mg / kg) by asingle ip injection. BALB / c mice were selected as donor mice and islet cells wereisolated from the mice. 500 islets were transplanted into recipient mice be-neath the capsule of the left kidney. Folding the islet transplantation the glu-cose from the mice was monitored and the rejection rate of islets was analyzed. Results: STZ could induce diabetes in the recipient mice within 1 week. Aftertransplantation of allograft islets, the increased glucose in wild-type (WT) mice returned to normal level and was maintained for 10 d. Unexpectedly, the rejection rate of islet allograft between IL-12p40-deficient mice and WT mice was similar. Conlusion: The results suggested that, although islet allo graft rejection is be-lieved to be Th1-cell predominant, the Th1 response inducer, IL-12 and IL-23 arenot essential to induce islet allograft rejection.