论文部分内容阅读
了解西藏自治区2012~2014年5岁以下健康儿童粪便标本中携带肠道病毒的血清型分布及流行特点。采集西藏自治区三地(拉萨、日喀则和山南地区)5岁以下健康儿童的粪便标本,同时收集相关流行病学信息。使用RD和L20B细胞进行病毒分离,将阳性分离株用肠道病毒分子定型法进行血清型鉴定。2012~2014年从西藏自治区5岁以下健康儿童中共采集了460份粪便标本(每名儿童采集1份),分离到肠道病毒共133株,包括脊髓灰质炎(脊灰)疫苗株33株,肠道病毒分离率为28.91%,非脊灰肠道病毒的分离率为21.74%。西藏自治区健康人群肠道病毒携带率和携带病毒的型别存在年代和地域的差异,但均以EV-B为主。其中在2012~2014年中,每年非脊灰肠道病毒谱中CV-B3是分离数量最多的。2012~2014年西藏5岁以下健康儿童中没有检出脊灰野病毒和脊灰疫苗衍生病毒,维持了无脊灰状态。检出了多种非脊灰肠道病毒,CV-B3一直在西藏自治区尤其是拉萨市健康儿童中隐性传播。本研究不仅为我国维持无脊灰工作提供坚实的实验室依据,同时,对非脊灰肠道病毒的流行本底的研究,为西藏自治区非脊灰肠道病毒的暴发提供预测预警信息和科技支撑。
To understand the serotype distribution and epidemic characteristics of enterovirus in stool specimens from healthy children aged under 5 years from 2012 to 2014 in the Tibet Autonomous Region. Stool samples from healthy children under 5 years of age were collected from three places (Lhasa, Shigatse and Shannan areas) in the Tibet Autonomous Region and relevant epidemiological information was collected. Viruses were isolated using RD and L20B cells, and positive isolates were serotyped using enteroviral molecular typing. 460 stool specimens (1 per child) were collected from healthy children under the age of 5 in Tibet Autonomous Region from 2012 to 2014. A total of 133 enteroviruses were isolated, including 33 polio (polio) vaccine strains, Enterovirus isolation rate was 28.91%, non-polio enterovirus isolation rate was 21.74%. The prevalence of enterovirus and carriage of virus in healthy population in Tibet Autonomous Region differed by age and region, but EV-B was the main factor. Among them, from 2012 to 2014, CV-B3 was the most abundant in the non-polio enterovirus spectrum. In 2012 ~ 2014, no poliovirus and poliovirus-derived virus were detected in healthy children under 5 years of age in Tibet, maintaining a polio-free status. A number of non-polio enteric viruses were detected, and CV-B3 has been implicitly transmitted among healthy children in the Tibet Autonomous Region, especially Lhasa. This study not only provides a solid laboratory basis for maintaining polio-free work in our country, but also studies the prevalence background of non-polio enterovirus and provides early warning information and technology for the outbreak of non-polio enterovirus in the Tibet Autonomous Region support.