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聚合酶链反应(Polymerase ChainReaction,简称PCR)是一种体外特异性扩增DNA的技术。它能将极微量的DNA样品特异地放大儿十万倍。目前国外已广泛应用PCR技术研究HBV感染。本次试验,我们应用北京医科大学肝病研究所提供的PCR扩增HBV基因试剂盒,对91例HBsAg慢性携带者血清进行PCR检测,探讨HBV感染者的传染性。
Polymerase Chain Reaction (PCR) is a technique that amplifies DNA in vitro. It can amplify extremely small amounts of DNA samples hundreds of thousands of times. At present, foreign countries have widely used PCR technology to study HBV infection. In this experiment, we applied PCR amplification of HBV gene kit provided by Institute of Hepatology, Beijing Medical University to detect the serum of 91 cases of chronic carriers of HBsAg and explore the contagion of HBV carriers.