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CO_2地质封存过程复杂,存在CO_2泄漏风险,进而会影响土壤生态系统,为研究地质封存CO_2泄漏应对措施,以玉米为研究对象,采用人工控制释放CO_2系统研究了玉米对地质封存CO_2泄漏的响应特征,并应用高通量测序技术分析了玉米土壤细菌群落结构及遗传多样性。结果表明:土壤CO_2浓度为30×104μL·L~(-1)(30 cm埋深处CO_2浓度)时,玉米表现出较好的适应能力,当CO_2泄漏量增加到40×104μL·L~(-1)以上时,玉米叶片等各项生长指标均表现为受胁迫特征;高通量测序结果显示试验共获得4个土壤样本的70 948个OTUs,但仅3.32%为4种泄漏条件下土壤所共有;遗传信息表明土壤细菌多样性和丰富度变化与CO_2泄漏浓度密切相关;变形菌门、拟杆菌门和酸杆菌门等为土壤优势菌群,而酸杆菌门在土壤CO_2浓度达40×104μL·L~(-1)下相对丰度大幅增加,暗示其可用作地质封存CO_2泄漏对土壤生态系统影响的指示菌。可见泄漏的CO_2会影响农作物生长及土壤细菌群落特征,而变化的土壤细菌多样性会影响农田生态系统功能等。
In order to study the countermeasures of CO2 sequestration in geologic sequestration, the CO2 sequestration of CO 2 system was studied by using controlled CO2 release system to study the response characteristics of maize to CO2 sequestration of geological sequestration , And analyzed the bacterial community structure and genetic diversity of corn by high-throughput sequencing. The results showed that maize showed good adaptability when soil CO_2 concentration was 30 × 104μL·L -1 (CO_2 concentration at depth of 30 cm). When CO2 leakage increased to 40 × 104μL·L ~ (-1) -1), all the growth indexes of maize leaves showed stress characteristics. High-throughput sequencing showed that 70 948 OTUs were obtained from 4 soil samples, but only 3.32% were soil under 4 conditions The genetic information showed that the variation of soil bacterial diversity and abundance was closely related to the concentration of CO 2 leakage. Proteobacteria, Bacteroidetes and Acidobacter were the dominant soil groups, while the acid bacteria in the soil CO 2 concentration reached 40 × The relative abundance at 104μL·L -1 increased significantly, suggesting that it could be used as an indicator of the impact of CO_2 leakage on soil ecosystem. It can be seen that CO 2 leaking can affect the growth of crops and the characteristics of soil bacterial communities. However, the diversity of soil bacteria affected the functions of farmland ecosystems.