目的 :采用反相高效液相色谱法测定龙胆泻肝丸中黄芩苷的含量。方法 :色谱柱为SpherisorbC1 8柱 ,流动相为甲醇 水 醋酸 (45∶55∶1 ) ,UV检测波长为 2 74nm。结果 :黄芩苷峰与其他组分峰的分离度为 3 .0 ,理论塔板数以黄芩苷峰计算为 2 3 32 0 ;方法的平均加样回收率为 98.6 % (n =5) ;黄芩苷在 0 .2 5～ 2 .5μg范围内 ,浓度与峰面积呈良好的线性关系。结论 :本法测定龙胆泻肝丸中黄芩苷的含量 ,结果准确 ,重复性好 Objective : To determine the baicalin content in Longdanxiegan Pills by RP-HPLC. Method: The chromatographic column was Spherisorb C18, and the mobile phase was methanol acetic acid (45:55:1). UV detection wavelength was 2 74nm. RESULTS: The separation of baicalin peak from other components peaks was 3.0, the theoretical plate number was baicalin peak calculated as 2 3 32 0, and the average recovery rate of the method was 98.6 % (n = 5); The glycoside ranged from 0.25 to 2.5 μg. The concentration and peak area showed a good linear relationship. Conclusion :This method was applied to the determination of baicalin in Longdanxiegan Pills with accurate results and good repeatability