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采用荧光光谱法、紫外可见吸收光谱法以及同步荧光光谱法研究了米格列奈钙与牛血清白蛋白(BSA)之间的相互作用。实验结果表明,米格列奈钙对牛血清白蛋白的荧光有较为明显的猝灭作用,其猝灭机制为动态猝灭,遵循Stern-Volmer方程;根据F9rster的非辐射能量转移理论,计算出米格列奈钙分子与牛血清白蛋白分子的结合距离为5.461nm;用同步荧光光谱技术探究了米格列奈钙对BSA构象的影响,结果发现,当激发和发射波长差为60nm时,荧光峰位发生了微小的蓝移,说明BSA色氨酸残基附近的外围微环境受到了米格列奈钙分子的影响,极性减弱,BSA的疏水性增强。
The interaction between mitiglinide and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, UV-Vis absorption spectroscopy and synchronous fluorescence spectroscopy. The experimental results show that the mitochondrial calcium on the fluorescence of bovine serum albumin has a more obvious quenching quenching mechanism for the dynamic quenching, following the Stern-Volmer equation; F9rster non-radiative energy transfer theory to calculate The binding distance between mitiglinide calcium and bovine serum albumin was 5.461nm. The effect of mitiglinide calcium on the conformation of BSA was explored by synchronous fluorescence spectroscopy. The results showed that when the difference between excitation and emission wavelength was 60nm, A slight blue shift occurred at the fluorescence peak, indicating that the peripheral microenvironment near the tryptophan residue of BSA is affected by the mitiglinide calcium molecule, the polarity is weakened, and the hydrophobicity of BSA is enhanced.