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甘蔗植物组织内的多酚类、色素类等物质是干扰ＲＡＰＤ扩增反应的主要成分。本研究以ＣＴＡＢ法和ＳＤＳ法为基础，着重对抗氧化剂（ＰＶＰ等）、酶（蛋白酶Ｋ、ＲＮａｓｅＡ）和酚抽提等因素对提取ＤＮＡ的质量及其ＲＡＰＤ的影响进行了比较筛选。结果表明：①甘蔗ＤＮＡ提取质量和纯度与ＲＮａｓｅＡ、抗氧化剂等处理直接相关，蛋白酶Ｋ、ＣＴＡＢ和ＳＤＳ的处理无明显差异；②对于甘蔗ＲＡＰＤ反应，模板ＤＮＡ中含有一定量的ＲＮＡ和蛋白质，其扩增产物无明显差异，但模板ＤＮＡ的降解程度和含有酚类、色素等是干扰甘蔗ＲＡＰＤ反应的主要因子。进一步分析讨论了甘蔗ＲＡＰＤ扩增的有效的ＤＮＡ提纯方法和影响因子。
Polyphenols, pigments and other substances in sugarcane plant tissues are the main components that interfere with the RAPD amplification reaction. Based on the CTAB method and the SDS method, we compared the effects of antioxidants (PVP, etc.), enzymes (Proteinase K, RNase A) and phenol extraction on the quality of extracted DNA and its RAPD. The results showed that: (1) The quality and purity of sugarcane DNA extraction were directly related to RNaseA and antioxidant treatment, and there was no significant difference in protease K, CTAB and SDS treatment; ②For RAPD reaction of sugarcane, the template DNA contained a certain amount of RNA and protein There was no significant difference in amplified product, but the degradation of template DNA and the presence of phenols and pigments were the main factors that interfere with RAPD reaction in sugarcane. Further analysis and discussion of sugarcane RAPD amplification of effective DNA purification methods and impact factors.