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应用差别ｐ Ｈ 值沉淀蛋白质的原理，建立了水稻条纹病毒病特异蛋白（ Ｓ Ｐ） 的两种提纯方法。这两种方法都可以从病叶中提纯到大量的 Ｓ Ｐ，其粗提纯量分别为０ ．８ 和２ ．０ ｍｇ／ｇ 病叶。通过 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 分离后得到了精提纯的蛋白，其分子量为２０ ．１ ｋ Ｄａ 。将粗提纯和精提纯的 Ｓ Ｐ 分别免疫兔子，制备出效价为５１ ２００ 和６ ４００ 的抗血清。将效价为６ ４００ 的高度特异性的抗血清用于研究 Ｒ Ｓ Ｖ Ｓ Ｐ 与 Ｒ Ｓ Ｖ Ｃ Ｐ 及同属的水稻草状矮化病毒（ Ｒ Ｇ Ｓ Ｖ） Ｓ Ｐ、 Ｃ Ｐ 之间的血清学关系，结果表明， Ｒ Ｓ Ｖ Ｓ Ｐ 的抗血清与 Ｒ Ｇ Ｓ Ｖ Ｃ Ｐ、 Ｒ Ｓ Ｖ Ｃ Ｐ 之间无反应，但可与 Ｒ Ｇ Ｓ Ｖ Ｓ Ｐ 微弱反应；而 Ｒ Ｇ Ｓ Ｖ Ｓ Ｐ、 Ｃ Ｐ 及 Ｒ Ｓ Ｖ Ｃ Ｐ 的抗血清与 Ｒ Ｓ Ｖ Ｓ Ｐ 之间都无血清学反应。结果证实了 Ｒ Ｓ Ｖ 和 Ｒ Ｇ Ｓ Ｖ 之间存在着进化上的亲缘关系
Two different purification methods of rice stripe virus disease-specific protein (S P) were established by using the principle of differential protein precipitation. Both of these methods can be purified from diseased leaves to a large amount of S P, with crude purifications of 0, respectively. 8 and 2. 0 mg / g diseased leaves. After purification by S D S P A G E purified refined protein, the molecular weight of 20. 1 k Da. The crude purified and purified purified S P were each immunized into rabbits to prepare antiserum with potencies of 51,200 and 6,400. The highly specific antiserum with a potency of 6 400 was used to study the relationship between R S V-S P and R S V-C P and the same R G S V S P, C P The results showed that the antiserum of RS-S P did not react with R G S V-C P and R S V-C P but reacted weakly with R G S V-S P ; While there was no serological reaction between R G S V S P, C P and R S V C P antiserum and R S V S P. The results confirm the evolutionary genetic relationship between R S V and R G S V