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为研究恶性、交界性、良性卵巢肿瘤囊内液中的血管内皮生长因子(VEGF)浓度和术前血清VEGF水平与肿瘤临床病理特征之间的关系,107例患者纳入研究,其中25例恶性,12例交界性,70例良性。手术切除的囊性卵巢肿瘤立即由一位妇科病理专家做出病理诊断,同时用无菌细针抽取囊内液,高速离心后收集上清液,-35℃保存备检。由一位不知病理结果的熟练技术员,按测定纤溶酶原激活剂系统内组分的常规步骤进行VEGF检测。该实验基于四个多克隆抗体以夹层形式结合的酶联免疫吸附试验,直接测定分子形式的VEGF。检测低限0.023 μg/L,具功能活性的是0.032 μg/L。血清CA 125采用自动AxSYM微粒酶免疫分析法检测。患者情况:术后残留病灶大小分≤2 cm或>2 cm;腹水或腹腔冲洗液有恶性细胞为阳性;术后随访期间发现肿瘤迹象为复发。统计分析采用Mann-Whitney u 检验和Spearman Rank
To study the relationship between the level of vascular endothelial growth factor (VEGF) and the serum level of VEGF in malignant, borderline and benign ovarian tumors and their clinicopathological features, 107 patients were included in the study, of which 25 were malignant, 12 cases of borderline, 70 cases of benign. The surgically resected cystic ovarian tumor was immediately diagnosed by a gynecological pathologist. The supernatant was collected after centrifugation at high speed and stored at -35 ° C. The VEGF assay was performed by a skilled technician with no known pathological results according to the usual procedures for determining components in the plasminogen activator system. This experiment directly measured the molecular form of VEGF based on the enzyme-linked immunosorbent assay (ELISA) of four polyclonal antibodies in sandwich format. The lower limit of detection was 0.023 μg / L and the functional activity was 0.032 μg / L. Serum CA 125 was detected using an automated AxSYM granzyme immunoassay. Patient’s condition: The size of residual lesion after operation was ≤2 cm or> 2 cm; the ascites or peritoneal washings were positive for malignant cells; and the signs of tumor were found to be recurrence during follow-up. Statistical analysis used the Mann-Whitney u test and Spearman Rank