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目的为适应大规模基因组扫描技术的需要，建立一种以ＴａｑＳｔａｒｔ抗体为酶保护剂、退火温度在一定范围内递降的多重ＰＣＲ体系。方法在５μｌ的总体积内，加５～１５对ＰＣＲ引物，用不同颜色的荧光标记（或相同荧光标记但其扩增片段相差５０ｂｐ以上），多个微卫星位点可同时得到扩增，并可通过ＤＮＡ测序仪进行电泳及数据分析。结果多个微卫星位点在５μｌ的体积内同时得到扩增。电泳条带图象清晰，获得了满意的基因分型结果。结论多重ＰＣＲ反应高产量、低成本，适用于人类基因组计划，尤其是基因作图、基因定位、法医学及人类进化等研究
ObjectiveTo meet the needs of large-scale genome scanning, a TaqStart antibody was used as enzyme protection reagent, and the annealing temperature decreased within a certain range of multiplex PCR system. Methods Five to fifteen pairs of PCR primers were added in a total volume of 5μl and labeled with different colors (or the same fluorescent marker but the amplified fragments were different by more than 50bp). Multiple microsatellite loci could be amplified at the same time. DNA sequencing by electrophoresis and data analysis. As a result, multiple microsatellite loci were simultaneously amplified in a volume of 5 μl. Electrophoretic band images were clear and satisfactory genotyping results were obtained. Conclusion Multiplex PCR reaction is high throughput and low cost and suitable for human genome project, especially gene mapping, gene mapping, forensic and human evolution