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在pH 8.5的Tris-HCl缓冲溶液中,钙黄绿素作为能量供体(D)可以与藏红T受体(A)发生有效的荧光共振能量转移(FRET),但加入六偏磷酸钠(SHMP)后,因其与受体发生静电作用破坏了该能量转移体系,使得荧光供体钙黄绿素荧光强度的增加(FD)与受体藏红T荧光强度的降低(FA)的比值(FD/FA)与SHMP浓度(cSHMP)呈良好的线性关系.基于此,建立了一种检测六偏磷酸盐的新方法.在优化条件下,该方法的检测范围为3.0×10 6~1.0×10 5mol/L,对6.0×10 6mol/L的六偏磷酸盐连续平行测定11次,其相对标准偏差(RSD)为3.1%.该方法具有选择性好、操作简单和检测速度快等优点,已成功应用于饮料中六偏磷酸钠的分析检测.
In the Tris-HCl buffer solution of pH 8.5, calcein as an energy donor (D) can undergo efficient fluorescence resonance energy transfer (FRET) with safranin T receptor (A), but sodium hexametaphosphate (SHMP) The energy transfer system is destroyed by the electrostatic interaction with the receptor, so that the ratio of the increase of the fluorescence intensity of the fluorescence donor calcein (FD) to the decrease of the fluorescence intensity of the receptor tangled T (FA) (FD / FA) And the concentration of SHMP (cSHMP) showed a good linear relationship.Therefore, a new method for the determination of hexametaphosphate was established.Under the optimized conditions, the detection range of this method was 3.0 × 10 6 ~ 1.0 × 10 5 mol / L , And its relative standard deviation (RSD) was 3.1% for the continuous parallel determination of 6.0 × 10 6 mol / L hexametaphosphate 11 times.The method has the advantages of good selectivity, simple operation and fast detection speed and has been successfully applied to Analysis of sodium hexametaphosphate in beverages.