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目的研究壬基酚对围产期雄性子代生殖系统PCNA和AROMATASE表达的影响,探讨壬基酚生殖发育毒性作用机制。方法对母鼠孕期第7~20D染毒壬基酚(NP)(50、100、200MG/KG),妊娠第20D和产后第2D分两批处死母鼠,测定血清雌二醇和血清NP浓度,并取雄性子代睾丸和附睾进行组织病理学观察和PCNA,ARO-MATASE免疫组织化学分析。结果实验组孕鼠血清雌二醇和血清NP浓度显著高于对照组,且呈剂量反应关系(P<0.05);妊娠第20D和产后第2D雄性子代睾丸的PCNA表达,100、200MG/KG NP染毒组低于对照组(P<0.05),且产后第2D雄性子代附睾PCNA表达和睾丸AROMATASE表达也低于对照组(P<0.05)。结论NP能干扰围产期雄性子代睾丸和附睾的正常生长发育。
Objective To study the effects of nonylphenol on the expression of PCNA and AROMATASE in the male offspring reproductive system and to explore the toxic mechanism of nonylphenol on reproductive development. Methods Twelve batches of rats were sacrificed at 7th to 20th day of gestation, and serum estradiol and serum NP levels were measured. Male and female testes and epididymis were taken for histopathological observation and PCNA, ARO-MATASE immunohistochemical analysis. Results The levels of serum estradiol and serum NP in the experimental group were significantly higher than those in the control group (P <0.05). The PCNA expression in the testis of the second and third male offspring in pregnancy was 100,200 MG / KG NP (P <0.05). The expression of PCNA and the expression of AROMATASE in epididymis of the second male offspring after birth were also lower than those of the control group (P <0.05). Conclusion NP can interfere with the normal growth and development of testes and epididymis in male offsprings.