论文部分内容阅读
离体保存是植物种质保存的重要手段之一,为实现对大花卷丹的保护性利用,本文对其组织培养体系及限制生长离体保存技术进行了研究。结果表明,在常温(23±2)℃、光照强度约为40μmol.m-2.s-1、光照时间14h.d-1的条件下,大花卷丹鳞片在MS+6-BA1.0mg.L-1+NAA0.2mg.L-1培养基中生长情况较好,能直接诱导芽,且小鳞茎的生长速度较快。将诱导出的小鳞茎切割后,接种到1/2MS+NAA0.5mg.L-1+活性炭2g.L-1生根培养基2～3周即能生根,生长状况良好。提高MS培养基中蔗糖达90和110g.L-1时可以抑制其生长,能够保存大花卷丹试管苗10个月,保存过程中生长正常,株高生长缓慢,但根长势较快。在蔗糖浓度90g.L-1基础上再添加30g.L-1甘露醇的培养基能进一步抑制试管苗根的生长。6个月后,转移到正常培养基上培养均能恢复生长,其鳞片在诱导培养基上能正常分化。因此,采用大花卷丹鳞片组织培养可以形成种苗,在培养基中添加高蔗糖浓度和甘露醇可以使其试管苗保存1年以上。
In vitro preservation is one of the important means of plant germplasm preservation. In order to realize the protective utilization of Cymbidium grandiflorum, this paper studied the tissue culture system and the technology of limiting the growth in vitro. The results showed that under the conditions of normal temperature (23 ± 2) ℃, light intensity of about 40μmol.m-2.s-1 and illumination time of 14h · d-1, The growth of L-1 + NAA0.2mg.L-1 medium was better, the shoots could be induced directly, and the growth of bulblets was faster. The induced bulblets were inoculated into 1 / 2MS + NAA0.5mg.L-1 + activated carbon 2g.L-1 rooting medium 2 to 3 weeks to root, good growth. Increasing sucrose up to 90 and 110g.L-1 in MS medium can inhibit its growth, save the large flower curcumin in vitro for 10 months, its growth is normal, the growth of its plant height is slow, its root growth is fast. In the sucrose concentration of 90g.L-1 based on the addition of 30g.L-1 mannitol medium can further inhibit the growth of test tube seedling roots. After 6 months, the cells were transferred to normal medium for growth and the scales were normalized on the induction medium. Therefore, the seedlings can be formed by the tissue culture of Magnolia officinalis, and the addition of high sucrose concentration and mannitol to the culture medium can save the seedlings of the seedlings for more than one year.