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目的 :利用已建立的高传能线密度 (ＬＥＴ)辐射诱导ＭＣＦ＿１０Ｆ细胞渐进性恶性转化模式 ,分析乳腺癌发展中特定表型阶段癌基因产物的表达水平。方法 :①指数生长的人乳腺上皮细胞株ＭＣＦ＿１０Ｆ经α粒子 (１５０ｋｅＶ/ μｍ)单次或两次 (６０ｃＧｙ/次 )照射 ,剂量率４．
OBJECTIVE: To investigate the progressive malignant transformation of MCF-10F cells by using established LET radiation and to analyze the expression of oncogene products of specific phenotypes during the development of breast cancer. Methods: ① exponential growth of human mammary epithelial cell line MCF_10F by α particles (150keV / μm) single or two (60cGy / times) irradiation, the dose rate of 4.