论文部分内容阅读
东北大黑鳃金龟(Holotrichia diomphalia Bates)是黑龙江省蛴螬优势种,为害严重,在旱田区、林区和园林苗圃中都有发生。近年来,在试验应用乳状菌防治蛴螬的探索中,因引进日本金龟(虫甲)芽孢杆菌(Bacillus popilliae var popilliae)对东北大黑鳃金龟不敏感,转而开始注意开发利用当地的乳状菌资源。在黑龙江省巴彦县龙泉林场,1978年秋、1982年春从自然感染乳状病的东北大黑鳃金龟幼虫中分离到A型和B型乳状芽孢杆菌各一株。四年来,对A型乳状芽孢杆菌进行了寄主范围、活体培养和离体培养、分类鉴定以及田间应用等方面的研究。致病力测定,该菌株对东北大黑鳃金龟的感病率高达81.8％,田间防治试验也取得了显著效果。
Holotrichia diomphalia Bates is a dominant species of 蛴 螬 in Heilongjiang Province. It is severely damaged and occurs in upland fields, forest areas and garden nurseries. In recent years, in the exploration of the application of the control and control of lactic acid bacteria, the introduction of the Japanese tortoise (Bacillus popilliae var popilliae) is not sensitive to the maculopaps of the North China, and began to pay attention to the development and utilization of the resources of the local lactic acid bacteria . In the Longquan Forest Farm, Bayan County, Heilongjiang Province, in autumn 1978 and in the spring of 1982, one type of Lactobacillus type A and B was isolated from the larvae of Chrysolophus gigas, which naturally infected with milk disease. In the past four years, the host range, in vitro and in vivo culture, classification and identification of Aspergillus niger A were studied. Pathogenicity determination, the strain of Northeast China black-headed beetle the highest prevalence of 81.8%, field control trials have also achieved remarkable results.