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在盐法破壁的基础上,添加质壁分离剂。当酵母浓度为10%、氯化钠浓度为5%时,添加5 mL乙酸乙酯,并在100℃下抽提2 h,可以增加核酸的提取率和纯度,使得提取率达到60%。
质壁分离剂盐法提取啤酒酵母RNA
【摘 要】
：
在盐法破壁的基础上,添加质壁分离剂。当酵母浓度为10%、氯化钠浓度为5%时,添加5 mL乙酸乙酯,并在100℃下抽提2 h,可以增加核酸的提取率和纯度,使得提取率达到60%。
【机 构】
：
大连工业大学,石家庄经济学校
【出 处】
：
河北化工
【发表日期】
：
2004年期
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