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目的:探讨西红花酸对脑缺血再灌注Caspase-3mRNA和NF-κB表达的影响。方法:SD大鼠随机分为6组,即假手术组、模型组、尼莫地平组(5 mg.kg-1)和西红花酸组(10,20,40 mg.kg-1)。假手术组、模型组给予等容量的生理盐水。各组大鼠每日上午灌胃给药1次,连续给药7 d。采用线栓法阻塞大脑中动脉制备局灶性脑缺血2 h再灌注22 h模型。观察大鼠神经功能缺陷评分、脑梗死体积,测定脑组织GSH-Px活性及Caspase-3mRNA和NF-κB表达。结果:西红花酸呈剂量依赖性的降低脑组织梗死体积、改善神经功能,增加脑组织GSH-Px活性,减少Caspase-3mRNA和NF-κB表达。结论:西红花酸可能通过增加GSH-Px活性,减少Caspase-3mRNA和NF-κB表达,保护脑缺血再灌注损伤。
Objective: To investigate the effect of crocetin on the expression of Caspase-3mRNA and NF-κB in cerebral ischemia-reperfusion rats. Methods: SD rats were randomly divided into 6 groups: sham operation group, model group, nimodipine group (5 mg.kg-1) and crocetin group (10, 20, 40 mg.kg-1). Sham operation group, model group given equal volume of saline. Rats in each group were orally administered once a day for consecutive 7 days. The occlusion of the middle cerebral artery was performed by occlusion of the middle cerebral artery (MCAO) to establish a model of focal cerebral ischemia for 2 hours and reperfusion for 22 hours. Neurological deficit score, volume of cerebral infarction, GSH-Px activity and expression of Caspase-3mRNA and NF-κB in brain were measured. Results: Crocetin could decrease the volume of cerebral infarction, improve neurological function, increase GSH-Px activity and decrease the expression of Caspase-3mRNA and NF-κB in a dose-dependent manner. Conclusion: Crocetin may protect the cerebral ischemia-reperfusion injury by increasing the activity of GSH-Px, decreasing the expression of Caspase-3 mRNA and NF-κB.