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应用抗人干细胞生长因子(SCF)单克隆抗体,通过化学偶联方法制备成亲和层析柱,用于纯化大肠杆菌表达的可溶性重组人rh-SCF.结果表明,经亲和柱纯化的样品经SDS-PAGE电泳检测纯度达95%以上,计算蛋白回收率为23.4%,并且纯化后rh-SCF生物活性明显高于纯化前样品.
The anti-human stem cell growth factor (SCF) monoclonal antibody was used to prepare an affinity chromatography column for the purification of soluble recombinant human rh-SCF expressed in E. coli by chemical coupling. The results showed that the purity of rh-SCF purified by affinity column was above 95% by SDS-PAGE and the calculated protein recovery was 23.4%. The biological activity of purified rh-SCF was significantly higher than that of the purified sample.