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利用实时定量RT-PCR技术,比较了不同时期小黑杨应拉木中与木材形成相关的基因及其转录因子的表达模式。研究结果表明,与纤维素合成相关的基因如纤维素合成酶基因(CesA)、蔗糖合成酶基因(SUSY)、ACC氧化酶基因(ACO)等基因胁迫后在弯曲茎的上侧中表达量高于其在弯曲茎的下侧中的表达;尤其是FLA基因家族在弯曲茎的上侧中大量表达,而在弯曲茎的下侧中几乎不表达。与之相反,与木质素合成相关的基因如咖啡酰辅酶A-O-甲基转移酶(CCoAOMT)、苯丙氨酸裂解酶(PAL)、漆酶(laccase)、过氧化物酶(POD)等基因在弯曲茎的上侧中表达量低而在弯曲茎的下侧中表达量高,转录因子MYB基因在弯曲茎的上侧中表达量高于其在弯曲茎的下侧中的。
Real-time quantitative RT-PCR was used to compare the expression patterns of wood-related genes and their transcription factors in Populus tomentosa at different stages. The results showed that the genes related to cellulose synthesis, such as CesA, SUSY and ACC oxidase genes, were highly expressed in the upper side of bent stem after stress In its expression in the lower side of the curved stem; in particular the FLA gene family is largely expressed in the upper side of the curved stem but scarcely expressed in the lower side of the curved stem. In contrast, genes related to lignin synthesis, such as genes of caffeoyl-CoA-methyltransferase (CCoAOMT), phenylalanine lyase (PAL), laccase and peroxidase (POD) The amount of expression in the upper side of the curved stem is low and the expression level is high in the lower side of the curved stem, and the transcription factor MYB gene is expressed higher in the upper side of the curved stem than in the lower side of the curved stem.