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目的:利用poly(lactide-co-glycolide)(PLGA)和poly(styrene-co-4-styrene-sulfonate)(PSS)制备带负电荷的牛血红蛋白纳米粒,并对其载氧性能和体外性能进行评价。方法:采用溶剂蒸发法制备出空白的PLGA-PSS的空白纳米粒,通过改变pH值,吸附牛血红蛋白,从而制备出牛血红蛋白PLGA纳米粒。通过TEM、粒径、Zeta电位、包裹率和载药量、体外释放及其携氧能力对该纳米粒进行了综合分析。结果:Hb-PLGA-PSS-NPsTEM电镜下呈类球形,平均粒径为226.8±23.4nm,Zeta电位为-68.62mV,优化条件后最大包裹率约为99.3%,载药量约为28.6%,在37℃,pH7.4的PBS溶液中释放缓慢。通过对Hb结构的分析表明此工艺未对蛋白的结构造成影响,体外携氧实验测量了P50(29mmHg)和Hill系数(2.036),结果说明该Hb纳米微囊具有良好携氧功能。结论:成功制备了一种Hb-PLGA-PSS-NPs纳米粒,稳定性好,具有很好的携氧能力。
OBJECTIVE: To prepare negatively charged bovine hemoglobin nanoparticles by poly (lactide-co-glycolide) (PLGA) and poly (styrene-co-4-styrene-sulfonate) Evaluation. Methods: Blank PLGA-PSS blank nanoparticles were prepared by solvent evaporation. The hemoglobin PLGA nanoparticles were prepared by changing the pH value and adsorbing the bovine hemoglobin. The nanoparticles were analyzed by TEM, particle size, Zeta potential, encapsulation efficiency and drug loading, in vitro release and oxygen carrying capacity. Results: The morphology of Hb-PLGA-PSS-NPsTEM was spherical under the electron microscope. The average particle size was 226.8 ± 23.4nm and the zeta potential was -68.62mV. The maximum encapsulation efficiency was about 99.3% and the drug loading was about 28.6% The release was slow at pH 7.4 in PBS at 37 ° C. Hb structure analysis showed that the process did not affect the structure of the protein. P50 (29mmHg) and Hill coefficient (2.036) were measured in oxygen-carrying experiments in vitro. The results show that the Hb nanocapsules have good oxygen carrying capacity. Conclusion: Hb-PLGA-PSS-NPs nanoparticles were successfully prepared with good stability and oxygen carrying ability.