This study presents an analysis of the spatial-temporal distribution of 230 archaeological sites in Guizhou Province, Southwest China for three selected time periods from the Paleolithic Age to the Shang-Zhou Dynasties. The relationship between archaeological sites distribution and environmental changes is also discussed based on paleo-environmental proxies of δ18O and δ13C recorded in stalagmites from Southwest China. The results show that: in the Paleolithic Age(260–10 ka BP), archaeological sites were concentrated in the central, northwestern and southwestern parts of Guizhou, where the high-altitudinal karst landforms with many natural caves suitable for human habitation are developed. In the Neolithic Age(10–3.6 ka BP), most of human settlements were concentrated in the central, northwestern and southwestern parts, while, a fewer sites were found on river terraces in the southern and eastern parts, and the intermontane basins in the central and western Guizhou. During the Shang-Zhou Dynasties(3.6–2.2 ka BP), the sites were mainly distributed in the intermontane basins and on river terraces, which were suitable for primitive aerial farming. The analysis of paleo-environmental proxies of δ18O and δ13C since 260 ka BP suggested that climate fluctuations had little impact on human settlements in this study area. The distinct physical environment, especially the spatial patterns of karst landforms and arable land played an important role in the archaeological sites distribution of Guizhou. This study presents an analysis of the spatial-temporal distribution of 230 archaeological sites in Guizhou Province, Southwest China for three selected time periods from the Paleolithic Age to the Shang-Zhou Dynasties. The relationship between archaeological sites distribution and environmental changes is also discussed based on paleo-environmental proxies of δ18O and δ13C recorded in stalagmites from Southwest China. The results show that: in the Paleolithic Age (260-10 ka BP), archaeological sites were concentrated in the central, northwestern and southwestern parts of Guizhou, where the high-altitudinal karst landforms with many natural caves suitable for human habitation are developed. In the Neolithic Age (10-3.6 ka BP), most of human settlements were concentrated in the central, northwestern and southwestern parts, while, a fewer sites were found on river terraces in the southern and eastern parts, and the intermontane basins in the central and western Guizhou. During the Sh The analysis was paleo-environmental proxies of δ18O and δ13C since 260 ka BP suggested (angiogenic Dynasties (3.6-2.2 ka BP), the sites were mainly distributed in the intermontane basins and on river terraces, which were suitable for primitive aerial farming. that climate fluctuations had this impact area this especially physical pattern distribution of Guizhou.