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本研究运用体视学原理,在光镜下对衰老大鼠睾丸各结构成份进行了定量观察研究,以探讨睾丸各结构成份年龄性变化与功能的关系。结果表明:1.随着年龄的增长,睾丸曲细精管的体密度(Vv)、表面积与体积之比(Sv/Vv)均明显降低(P<0.01);而线密度(Lv)则无明显变化(P>0.05)。2.老年组曲细精管的生精细胞除精原细胞的体密度(Vv)明显降低外,初级精母细胞和精细胞的体密度(Vv)均有较大的增加,差异显著(P<0.01)。3.间质体密度(Vv)、曲细精管基膜调和平均厚度(th)两个参数值在衰老大鼠睾丸中增大,间质中毛细血管的线密度(Lv)降低(P<0.01)。上述测量结果提示:衰老大鼠睾丸曲细精管的萎缩退化主要与精细胞发育障碍有关。此外,由于间质纤维化、曲细精管基膜调和平均厚度增大造成生精细胞在发育过程中营养物质的缺乏。
In this study, the principle of stereology was used to observe quantitatively the structure of testis in aging rats under the light microscope to explore the relationship between age-related changes in testicular structure and function. The results showed that: (1) With age, testicular seminiferous tubules had significantly lower Vv, Sv / Vv (P <0.01), while no linear density (Lv) Significant changes (P> 0.05). Spermatogenic cells in the seminiferous tubules of the aged group showed significantly higher body density (Vv) than that of the spermatogonia (P < 0.01). The two parameters, Vs and TH of seminiferous tubules increased in testis of aging rats, while the linear density (Lv) of capillary in interstitial decreased (P < 0.01). The above results suggest that the atrophic and degenerative changes of testicular seminiferous tubules in aging rats are mainly related to the development of spermatocyte dysplasia. In addition, due to interstitial fibrosis, seminiferous tubules basement membrane reconcile the average thickness of the growth of spermatogenic cells in the development of nutrients caused by the lack of.