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为明确小麦抗锈种质南农790条锈病抗性特征和抗病遗传规律,以5个条锈菌生理小种CYR23、CYR29、CYR31、CYR32和CYR33对其进行苗期抗谱鉴定,并以CYR32和CYR33混合小种对南农790、Avocet S及南农790与Avocet S正交所获得的F1代、F2代和F2∶3群体进行混合小种成株期抗条锈性鉴定及遗传分析。抗病鉴定结果显示,南农790苗期对CYR32和CYR33表现为中度感病(IT:6~7),对其余小种表现为抗病(IT≤2),成株期对混合小种表现为近免疫(IT≤2,S≤5%),表明南农790具有成株期抗条锈性;各世代抗病遗传分析结果表明,其成株期抗病性由一个显性单基因控制,暂命名为YrNan;采用集群分离分析法(BSA),以SSR分子标记对F2代分离群体进行分子作图,发现了6个与YrNan连锁的SSR标记(Xgwm11,Xbarc187,Xbarc181,Xgwm273,Xwmc419和Xwmc216),并将其定位于小麦的1BL染色体,两侧最近的标记分别为Xbarc181(1.3cM)和Xgwm273(6.3cM)。研究结果为南农790抗条锈病基因的分子标记辅助选择及在育种上的应用提供了依据。
In order to clarify the resistance characteristics and resistance inheritance of 790 rust resistant wheat cultivars Nannong 790 in wheat, anti-rust germplasms were identified with 5 stripelet races CYR23, CYR29, CYR31, CYR32 and CYR33, CYR32 and CYR33 mixed races F1 hybrids, F1 F2 hybrids and F2 F2 hybrids obtained from orthogonal crosses between Nannong 790, Avocet S, Nannong 790 and Avocet S were identified for their resistance to stripe rust and their genetic analysis . The results of disease resistance identification showed that Nannong 790 showed moderate susceptibility to CYR32 and CYR33 at the seedling stage (IT: 6 ~ 7), resistance to the rest of the races (IT≤2) (IT≤2, S≤5%), indicating that Nannong 790 has the resistance to stripe rust at the adult stage. The genetic analysis of disease resistance in each generation showed that its resistance to disease at the adult stage consisted of a dominant single gene (Xgwm11, Xbarc187, Xbarc181, Xgwm273, Xwmc419, Xgwm273 and Xwmc419) were sequenced by cluster analysis (BSA) and SSR molecular markers on the F2 population. And Xwmc216) and mapped to chromosome 1BL of wheat. The nearest markers on both sides were Xbarc181 (1.3cM) and Xgwm273 (6.3cM), respectively. The results provided the basis for molecular marker-assisted selection of Nannong 790 stripe rust resistance gene and its application in breeding.