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目的探讨外周血清过氧化物酶(myeloperoxidase,MPO)、中性粒细胞(neutrophils,PMN)及单核细胞趋化蛋白-4(monocyte chemoattractant protein-4,MCP-4)在小儿支气管哮喘的血清水平,为临床有效防治提供有效参考。方法采用酶联免疫吸附法分别测定67例支气管哮喘患儿(哮喘组)和30例健康儿童(正常对照组)血清MPO、PMN及MCP-4水平,并进行比较。结果 1)MPO的血清水平在支气管哮喘急性发作期为[(878.43±18.77)U/L]高于哮喘缓解组[(678.62±33.36)U/L]及健康对照组[(703.17±18.26)U/L](P均<0.05);2)MCP-4在支气管哮喘急性发作期患儿血清水平[(117.34±18.77)pg/mL]高于哮喘缓解组[(55.67±6.13)pg/mL]及健康对照组[(33.74±2.98)pg/mL](P均<0.05)。3)PMN绝对值在支气管哮喘急性发作期[(10.31±1.98)×109/L]高于哮喘缓解组[(4.63±1.51)×109/L]及健康对照组[(4.82±1.51)×109/L](P均<0.05)。MPO、PMN及MCP-4的升高程度与病情严重程度相关,且两者之间呈正相关(r=0.976,0.989;P均<0.01)。结论 MPO、PMN及MCP-4在支气管哮喘中发挥了重要作用,与气道炎症反应有关,血清MPO、PMN及MCP-4可做为支气管哮喘的生化检测指标。哮喘缓解期仍存在慢性炎症反应。
Objective To investigate the serum levels of peripheral blood serum peroxidase (myeloperoxidase, MPO, neutrophils (PMN) and monocyte chemoattractant protein-4 (MCP-4) , Provide a valid reference for effective clinical control. Methods Serum levels of MPO, PMN and MCP-4 in 67 asthmatic children (asthma group) and 30 healthy children (normal control group) were determined by enzyme-linked immunosorbent assay and compared. Results 1) The serum level of MPO was significantly higher in acute exacerbation of bronchial asthma (878.43 ± 18.77 U / L) than in asthma remission group (678.62 ± 33.36 U / L) and healthy control group (703.17 ± 18.26) U (P <0.05); 2) The serum level of MCP-4 in patients with acute exacerbation of bronchial asthma [(117.34 ± 18.77) pg / mL] was higher than that in the asthma remission group [(55.67 ± 6.13) pg / And healthy control group [(33.74 ± 2.98) pg / mL] (all P <0.05). 3) The absolute value of PMN was significantly higher in acute exacerbation of bronchial asthma (10.31 ± 1.98 × 109 / L) than in asthma remission group (4.63 ± 1.51) × 109 / L and (4.82 ± 1.51) × 109 / L] (all P <0.05). The levels of MPO, PMN and MCP-4 were correlated with the severity of the disease, and there was a positive correlation between them (r = 0.976,0.989; P <0.01). Conclusions MPO, PMN and MCP-4 play an important role in bronchial asthma and are related to airway inflammation. Serum MPO, PMN and MCP-4 can be used as biochemical markers of bronchial asthma. There are still chronic inflammatory reactions in asthma remission.