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1970年,Wildenthal首次建立起哺乳动物胎心培养技术,并能使培养的心脏存活和跳动达4～8天后,近年来,以离体培养的心脏进行心脏病的基础理论和临床应用研究越来越多。但国内未见报导。基于研究需要,我们建立起小鼠胎心培养方法,并做了电镜观察。无菌下,借助于解剖显微镜,完整无损地摘出16～20天胎鼠心脏,放入培养瓶内气体和液体交界面的不锈钢支持网上。瓶口密封。培养液为Eagle+35％小牛血清+2mg/ml葡萄糖。气体为95％O_2+5％CO_2。两天换一次气体和液体。37℃恒温中培养。定期做电镜观察。本实验采用的培养技术能维持小鼠整体胎心存活和自主性搏动达27±2.3(SD)天。房室节率通常在140～160次/分。电镜观察表明,培养1～3天胎心显示基本正常的超微结
In 1970, Wildenthal first established mammalian fetal heart rate culture technology, and can make the cultured heart survive and beating for 4 to 8 days. In recent years, the basic theory and clinical application research of heart in vitro culture have been more and more more. However, no reports of domestic. Based on the research needs, we established a mouse fetal heart rate culture method, and made electron microscopy. Sterile, with the help of a dissecting microscope, completely intact extracted 16 to 20 days fetal rat heart, into the flask gas and liquid interface stainless steel support network. Bottle sealed. The medium was Eagle + 35% calf serum + 2 mg / ml glucose. The gas is 95% O_2 + 5% CO_2. Change gas and liquid every other day. 37 ℃ constant temperature in culture. Regular electron microscopy. The culture technique used in this experiment can maintain the overall fetal heartbeat survival and spontaneous pulsation of mice up to 27 ± 2.3 (SD) days. Atrioventricular node rate is usually 140 to 160 beats / min. Electron microscopy showed that the culture of fetal heart rate 1 to 3 days showed normal normal ultrastructure