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利用偏钒酸铵氧化儿茶酚胺化合物的作用,采用容量法和分光光度法测定肾上腺素、去甲肾上腺素、异丙基去甲肾上腺素、甲基多巴等儿茶酚胺化合物及其制剂,取得满意的结果。这些儿茶酚胺化合物氧化生成的化合物可在485mm处用分光光度法测定。肾上腺素线性范围在0.09～0.90 mg,去甲肾上腺素在0.07～0.65mg,异丙基去甲肾上腺素在0.07～0.75 mg,甲基多巴在0.10～0.95mg。一、试剂: 0.02 M偏钒酸铵(NH_4VO_3)标准溶液。0.02 M肾上腺素、去甲肾上腺素、异丙基去甲肾上腺素、甲基多巴溶液。
The use of ammonium metavanadate oxidation of catecholamines role, the volumetric method and spectrophotometric determination of epinephrine, norepinephrine, isopropyl norepinephrine, methyldopa and other catecholamines and their preparations, and achieved satisfactory results . The compounds produced by oxidation of these catecholamine compounds can be determined spectrophotometrically at 485 mm. The linear range of epinephrine was 0.09 ~ 0.90 mg, norepinephrine 0.07 ~ 0.65 mg, isopropyl norepinephrine 0.07 ~ 0.75 mg, methyldopa 0.10 ~ 0.95 mg. First, the reagents: 0.02 M ammonium metavanadate (NH_4VO_3) standard solution. 0.02 M epinephrine, norepinephrine, isopropyl norepinephrine, methyldopa solution.