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0 引言胆管细胞癌目前是一种不可治愈性的恶性肿瘤,早期诊断率低,治疗手段主要包括手术、化疗、放疗,但疗效均不够理想.许多患者的死亡原因并不是肿瘤复发转移,而是胆道梗阻,因此提高局部肿瘤治疗的效率,保证胆道通畅是目前提高胆管癌生存率的有效方法.自杀基因疗法是有效治疗肿瘤且有临床应用潜能的治疗策略之一.本研究利用重组逆转录病毒载体,通过脂质体介导将βG 基因转染人胆管癌细胞株QBC939,用其不同浓度的前体药-葡萄糖醛酸化表阿霉素[Epi-bus]处理,探索βG 基因/βG 前体药系统对人胆管癌细胞的杀伤作用及旁观者效应,从而建立一种新的自杀基因系统.
0 Introduction Cholangiocarcinoma is currently an incurable malignancy. The early diagnosis rate is low. The treatment methods mainly include surgery, chemotherapy, and radiotherapy. However, the curative effect is not ideal. The cause of death in many patients is not the recurrence and metastasis of the tumor. Biliary obstruction, so to improve the efficiency of local tumor treatment and ensure biliary tract patency is currently an effective method to improve the survival rate of cholangiocarcinoma. Suicide gene therapy is one of the treatment strategies for effective treatment of tumors and has clinical potential. This study uses recombinant retroviruses. Vectors were transfected with βG gene into human cholangiocarcinoma cell line QBC939 by liposome-mediated transfection, and were treated with different concentrations of prodrug-Epi-bus to explore βG gene/βG precursor. The killing effect of the drug system on human cholangiocarcinoma cells and the bystander effect, thus establishing a new suicide gene system.