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目的神经系统和免疫系统之间存在广泛的相互作用。许多研究表明,细菌脂多糖(lipopolysaccharide,LPS)或白介素1β引起的免疫激活会造成学习记忆的损伤。但也有一些研究报道免疫激活对学习记忆几乎没有影响。因此,免疫激活与学习记忆损伤之间的关系尚不十分清楚。本研究旨在检测LPS对空间记忆提取的影响,探讨免疫激活与记忆之间的相互作用。方法先在水迷宫中训练8周龄C57BL/6J系小鼠,随后小鼠腹腔注射LPS并进行空间觅向能力测试。记录小鼠在目标象限游泳的时间和穿过原平台位置的次数,以判断动物记忆提取再现能力。用酶联免疫法检测小鼠海马中白介素1β的水平。结果 LPS能引起小鼠产生明显的病态行为,并导致海马中白介素1β的水平显著升高。注射LPS的小鼠在目标象限游泳的时间以及穿越逃生台位置的次数与对照组小鼠相比没有显著差异。结论 LPS引起的免疫激活并不损害空间记忆的提取。
Purpose There is a wide range of interactions between the nervous system and the immune system. Numerous studies have shown that immune activation by bacterial lipopolysaccharide (LPS) or interleukin-1β causes learning and memory impairments. However, some studies have reported that immune activation has almost no effect on learning and memory. Therefore, the relationship between immune activation and learning and memory impairment is not yet clear. The purpose of this study was to examine the impact of LPS on spatial memory retrieval and to explore the interaction between immune activation and memory. Methods Eight-week-old C57BL / 6J mice were trained in a water maze, followed by intraperitoneal injection of LPS and spatial targeting ability test. Record mice swimming time in the target quadrant and the number of times through the original platform position to determine the animal memory retrieval ability. The levels of interleukin-1β in the hippocampus of mice were detected by enzyme-linked immunosorbent assay. Results LPS induced obvious morbid behavior in mice and resulted in a significant increase of interleukin-1β in the hippocampus. There was no significant difference in the number of mice injected with LPS swimming in the target quadrant and the number of crossover stations compared to control mice. Conclusion The immune activation induced by LPS does not impair the extraction of spatial memory.