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为维持中国无脊髓灰质炎(脊灰)状态及疫苗免疫策略的转变提供科学依据,本文对中国(不包括港澳台地区,以下同)2010年从急性弛缓性麻痹(Acute flaccid paralysis,AFP)病例监测系统分离的123株非野毒Ⅱ型脊灰病毒(Po-liovirus,PV)进行基因特征分析。利用逆转录-聚合酶链反应(Reverse transcription-polymerase chain reaction,RT-PCR)扩增VP1编码区,并对扩增产物进行序列测定,测序结果经Sequencher 4.8、BioEdit 7.0.9和MEGA 5.0软件分析。结果显示2010年未发现脊灰野病毒(Wild poliovirus,WPV)。65株PV在VP1编码区nt2 909发生突变,同时该位点又是已知的神经毒力决定位点。从云南省分离到2株疫苗衍生脊灰病毒(Vaccine-derived poliovirus,VDPVs),经流行病学和实验室检测结果分析表明,这2株都不同于以往发现的VDPVs,是新发现的VDPVs。建议加强全国AFP病例的流行病学和实验室监测和检测,为及时发现VDPVs传播和WPV的输入性暴发提供科学依据。
To provide a scientific basis for maintaining the status of poliomyelitis-free (polio) in China and the change of vaccine immunization strategies, this paper analyzes the cases of Acute flaccid paralysis (AFP) in China (excluding Hong Kong, Maucao and Taiwan) 123 strains of non-virulent poliovirus (Po-liovirus) isolated from surveillance system were analyzed for their genetic characteristics. VP1 coding region was amplified by reverse transcription-polymerase chain reaction (RT-PCR), and the amplified products were sequenced. Sequencing results were analyzed by Sequencher 4.8, BioEdit 7.0.9 and MEGA 5.0 software . The results showed that in 2010, no wild poliovirus (WPV) was found. Sixty-five PVs mutated at nt2 909 in the VP1 coding region, and this site is again known as a site of neurovirulence determinations. Two vaccine-derived polioviruses (VDPVs) were isolated from Yunnan Province. The epidemiological and laboratory results showed that the two strains were different from the previously found VDPVs and were newly discovered VDPVs. It is suggested to strengthen the epidemiological and laboratory monitoring and testing of AFP cases nationwide and provide a scientific basis for timely detection of VDPVs transmission and WPV input outbreaks.